It features five larger-than-life sculptures of characters from the ever-popular books created by Julia Donaldson and Axel Scheffler: Mouse, Fox, Owl, Snake and the Gruffalo.
The trail will be open every day with the aim of encouraging children to enjoy the great outdoors and adventure into the woods.
The characters were made by Garry Turler, a chainsaw art and wood sculptor from Cwmtwrch, in the Swansea Valley.
Garry, who runs Wildboar Carvings, said: “I’m extremely happy to have been asked to create the Gruffalo and other creatures for the new Gruffalo Trail at the National Botanic Garden.
“I’ve had to keep quiet about this for a while but I can now say where they are going.”
The Garden’s Head of Marketing, David Hardy said: “The craftsmanship is incredible and helps bring to life this fabulous and much-loved tale. We are sure they will a big hit and a massive draw to this wonderful garden.”
The National Botanic Garden is open from 10am every day.
For more information, visit botanicgarden.wales